微泡
转移
血管生成
肿瘤微环境
癌症研究
生物
蛋白质组
转移性乳腺癌
癌细胞
癌症
细胞生物学
乳腺癌
小RNA
生物信息学
生物化学
肿瘤细胞
基因
遗传学
作者
Lahiru Gangoda,Michael Liem,Ching‐Seng Ang,Shivakumar Keerthikumar,Christopher G. Adda,Belinda S. Parker,Suresh Mathivanan
出处
期刊:Proteomics
[Wiley]
日期:2017-12-01
卷期号:17 (23-24)
被引量:110
标识
DOI:10.1002/pmic.201600370
摘要
Cancer cells actively release extracellular vesicles, including exosomes, into the surrounding microenvironment. Exosomes play pleiotropic roles in cancer progression and metastasis, including invasion, angiogenesis, and immune modulation. However, the proteome profile of exosomes isolated from cells with different metastatic potential and the role of these exosomes in driving metastasis remains unclear. Here, we conduct a comparative proteomic analysis of exosomes isolated from several genetically related mouse breast tumor lines with different metastatic propensity. The amount of exosomes produced and the extent of cancer-associated protein cargo vary significantly between nonmetastatic and metastatic cell-derived exosomes. Metastatic cell-derived exosomes contain proteins that promote migration, proliferation, invasion, and angiogenesis while the nonmetastatic cell-derived exosomes contain proteins involved in cell-cell/cell-matrix adhesion and polarity maintenance. The metastatic exosomes contain a distinct set of membrane proteins including Ceruloplasmin and Metadherin which could presumably aid in targeting the primary cancer cells to specific metastatic sites. Hence, it can be concluded that the exosomes contain different protein cargo based on the host cells metastatic properties and can facilitate in the dissemination of the primary tumors to distant sites.
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