生物相容性
生物陶瓷
材料科学
涂层
乳酸脱氢酶
生物医学工程
扫描电子显微镜
再生(生物学)
雪旺细胞
化学
医学
纳米技术
解剖
复合材料
生物化学
冶金
生物
酶
细胞生物学
作者
Quan Yuan,He Gong,Zhi Tan,Ping Gong,Xiao Yu Li,Zhi Qing Chen,Yu Ban,Hua Tang,Fang Fang Wang,Yi Man,Na Wei,Zhenhong Zhu
出处
期刊:Key Engineering Materials
日期:2007-02-01
卷期号:330-332: 1393-1396
被引量:6
标识
DOI:10.4028/www.scientific.net/kem.330-332.1393
摘要
Sensory perception thresholds of implants were much higher than those of natural teeth. The purpose of this study was to evaluate the biocompatibility of TiO2/HA-biocoated dental implants for nerve regeneration using cultured Schwann cells. The nano-TiO2/HA composite bioceramic coating was developed on the surfaces of commercially pure titanium discs by sol-gel route, and characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Using smooth titanium discs as a control, Schwann cell responses to the coating were evaluated by SEM, MTT assay, total protein content and leakage of cytosolic lactate dehydrogenase (LDH) activity. In addition, the amount of brain-derived neurotrophic factor (BDNF) secreted by Schwann cells was measured by Enzyme-Linked Immunosorbent Assay (ELISA). It was observed that the coating had fine crystallites and homogeneous micropores in the diameter of 5-10 um. The cell morphology, proliferation and function were unaffected. The results indicate that the TiO2/HA bioceramic coating developed on the surface of pure titanium by sol-gel route had good biocompatibility with Schwann cells for nerve regeneration around dental implants.
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