中和
病毒学
病毒
抗血清
吞吐量
抗体
呼吸系统
生物
医学
免疫学
计算机科学
电信
解剖
无线
作者
Lien Anh Ha,Reuben Tse,Jordan Nathanielsz,Jeremy Anderson,Darren Suryawijaya Ong,Keith J. Chappell,Kim Mulholland,Paul V. Licciardi
摘要
Respiratory syncytial virus-specific neutralizing antibodies (RSV NAbs) are an important marker of protection against RSV. A number of different assay formats are currently in use worldwide so there is a need for an accurate and high-throughput method for measuring RSV NAbs. We describe here an imaging-based micro-neutralization assay that has been tested on RSV subgroup A and can also be adapted for RSV subgroup B and different sample types. This method is highly reproducible, with inter-assay variations for the reference antiserum being less than 10%. We believe this assay can be readily established in many laboratories worldwide at relatively low cost. Development of an improved, high-throughput assay that measures RSV NAbs represents a significant step forward for the standardization of this method internationally as well as being critical for the evaluation of novel RSV vaccine candidates in the future.
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