MP28-18 DISSECTING THE TRANSCRIPTOME LANDSCAPE OF MOUSE BLADDER UROTHELIUM BY SINGLE-CELL RNA-SEQ ANALYSIS

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology I (MP28)1 Apr 2020MP28-18 DISSECTING THE TRANSCRIPTOME LANDSCAPE OF MOUSE BLADDER UROTHELIUM BY SINGLE-CELL RNA-SEQ ANALYSIS Yan Li*, Benkang Shi, and Yaxiao Liu Yan Li*Yan Li* More articles by this author , Benkang ShiBenkang Shi More articles by this author , and Yaxiao LiuYaxiao Liu More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000867.018AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Much of the information to date in terms of subtypes and function of bladder urothelial cells were derived from anatomical location or by the expression of a small number of marker genes. To have a comprehensive map of the cellular anatomy of bladder urothelial cells, we performed single-cell RNA-sequencing to thoroughly characterize mouse bladder urothelium. METHODS: 18,917 single cells from C57BL/6 mouse bladder urothelium was analyzed by unbiased single-cell RNA sequencing to classify bladder cell types and their underlying functions. RESULTS: Clustering analysis identified 8 distinct cell clusters from mouse bladder urothelial cells. The homology and heterogeneity of mouse bladder urothelial cell types were compared and both conservative and heterogeneous aspects of mouse bladder urothelial cell evolution were identified. Two different types of umbrella cells were distinguished by the marker gene expression of F3, Plk2, Scube2, and Myh14. We also discovered a novel type of bladder urothelial cells marked by Plxna4 that may be involved with collective migration of epithelial cells during the epithelial wound healing. We verify these Plxna4+ epithelial cells also occur in human bladders. We also found a number of bladder pain syndrome-regulating genes differentially expressed among different subtypes. CONCLUSIONS: Our study provides a comprehensive characterization of bladder urothelial cells, which is fundamental to understanding bladder urothelium biology and bladder diseases. Source of Funding: National Natural Science Foundation of China (Grant No. 81470987, 81900637) © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e426-e427 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yan Li* More articles by this author Benkang Shi More articles by this author Yaxiao Liu More articles by this author Expand All Advertisement PDF downloadLoading ...