染色质
生物
转座酶
计算生物学
转录因子
嘉雅宠物
DNA测序
核小体
遗传学
DNA
基因
基因组
芯片排序
转座因子
作者
Yuanyuan Sun,Nan Miao,Tao Sun
出处
期刊:Hereditas
[Springer Nature]
日期:2019-08-15
卷期号:156 (1)
被引量:46
标识
DOI:10.1186/s41065-019-0105-9
摘要
Chromatin accessibility is crucial for gene expression regulation in specific cells and in multiple biological processes. Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq) is an effective way to reveal chromatin accessibility at a genome-wide level. Through ATAC-seq, produced reads from a small number of cells reflect accessible regions that correspond to nucleosome positioning and transcription factor binding sites, due to probing hyperactive Tn5 transposase to DNA sequence.In this review, we summarize both principle and features of ATAC-seq, highlight its applications in basic and clinical research. ATAC-seq has generated comprehensive chromatin accessible maps, and is becoming a powerful tool to understand dynamic gene expression regulation in stem cells, early embryos and tumors.
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