摘要
No AccessJournal of UrologyReview Article1 Nov 2013Drosophila melanogaster as an Emerging Translational Model of Human Nephrolithiasis Joe Miller, Thomas Chi, Pankaj Kapahi, Arnold J. Kahn, Man Su Kim, Taku Hirata, Michael F. Romero, Julian A.T. Dow, and Marshall L. Stoller Joe MillerJoe Miller University of California-San Francisco, San Francisco, California Current address: Department of Urology, Fort Belvoir Community Hospital, Fort Belvoir, Virginia 22060. More articles by this author , Thomas ChiThomas Chi University of California-San Francisco, San Francisco, California More articles by this author , Pankaj KapahiPankaj Kapahi Buck Institute for Research on Aging, Novato, California More articles by this author , Arnold J. KahnArnold J. Kahn Buck Institute for Research on Aging, Novato, California More articles by this author , Man Su KimMan Su Kim Buck Institute for Research on Aging, Novato, California More articles by this author , Taku HirataTaku Hirata Mayo Clinic College of Medicine, Rochester, Minnesota More articles by this author , Michael F. RomeroMichael F. Romero Mayo Clinic College of Medicine, Rochester, Minnesota More articles by this author , Julian A.T. DowJulian A.T. Dow University of Glasgow, Glasgow, United Kingdom More articles by this author , and Marshall L. StollerMarshall L. Stoller University of California-San Francisco, San Francisco, California Financial interest and/or other relationship with Ravine, EMKinetics, Bard, Boston Scientific and Cook. More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.03.010AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: The limitations imposed by human clinical studies and mammalian models of nephrolithiasis have hampered the development of effective medical treatments and preventive measures for decades. The simple but elegant Drosophila melanogaster is emerging as a powerful translational model of human disease, including nephrolithiasis. It may provide important information essential to our understanding of stone formation. We present the current state of research using D. melanogaster as a model of human nephrolithiasis. Materials and Methods: We comprehensively reviewed the English language literature using PubMed®. When necessary, authoritative texts on relevant subtopics were consulted. Results: The genetic composition, anatomical structure and physiological function of Drosophila malpighian tubules are remarkably similar to those of the human nephron. The direct effects of dietary manipulation, environmental alteration and genetic variation on stone formation can be observed and quantified in a matter of days. Several Drosophila models of human nephrolithiasis have been developed, including genetically linked and environmentally induced stones. A model of calcium oxalate stone formation is among the most recent fly models of human nephrolithiasis. Conclusions: The ability to readily manipulate and quantify stone formation in D. melanogaster models of human nephrolithiasis presents the urological community with a unique opportunity to increase our understanding of this enigmatic disease. References 1 : The insect nephrocyte is a podocyte-like cell with a filtration slit diaphragm. Nature2009; 457: 322. 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Google Scholar © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byAssimos D (2020) Re: Targeted Renal Knockdown of Na+/H+ Exchanger Regulatory Factor Sip1 Produces Uric Acid Nephrolithiasis in DrosophilaJournal of Urology, VOL. 204, NO. 1, (179-179), Online publication date: 1-Jul-2020. Volume 190Issue 5November 2013Page: 1648-1656 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.Keywordsanimalkidneydisease modelsDrosophila melanogasternephrolithiasismalpighian tubulesAcknowledgmentsRichmond Lee created figures 1, 4 and 6.MetricsAuthor Information Joe Miller University of California-San Francisco, San Francisco, California Current address: Department of Urology, Fort Belvoir Community Hospital, Fort Belvoir, Virginia 22060. More articles by this author Thomas Chi University of California-San Francisco, San Francisco, California More articles by this author Pankaj Kapahi Buck Institute for Research on Aging, Novato, California More articles by this author Arnold J. Kahn Buck Institute for Research on Aging, Novato, California More articles by this author Man Su Kim Buck Institute for Research on Aging, Novato, California More articles by this author Taku Hirata Mayo Clinic College of Medicine, Rochester, Minnesota More articles by this author Michael F. Romero Mayo Clinic College of Medicine, Rochester, Minnesota More articles by this author Julian A.T. Dow University of Glasgow, Glasgow, United Kingdom More articles by this author Marshall L. Stoller University of California-San Francisco, San Francisco, California Financial interest and/or other relationship with Ravine, EMKinetics, Bard, Boston Scientific and Cook. More articles by this author Expand All Advertisement PDF downloadLoading ...