GO-PtNi hybrid nanoenzyme mimics for the colorimetric detection of hydrogen peroxide

GO-PtNi nanocomposites with intrinsic peroxidase-like activity were obtained through one-pot synthesis. The hybrid nanomaterial was characterized by transmission electron microscopy (TEM) and X-ray powder diffraction (XRD). The peroxidase-like activity of GO-PtNi nanocomposites was found to be dependent on pH, temperature, the concentration of enzyme and substrates. The optimal conditions for the catalytic activity of GO-PtNi nanocomposites were determined. Based on these findings, a simple and sensitive colorimetric method for the detection of H2O2 by using GO-PtNi nanocomposites and 3,3′,5,5′-tetramethylbenzidine (TMB) was developed. The linear range was from 0.08 to 1.5 mM with a detection limit of 5 μM.