铝元素
后转座子
遗传学
序列(生物学)
基因组
DNA测序
重复序列
人类基因组
计算生物学
分子生物学
DNA
生物
转座因子
基因
出处
期刊:Methods in molecular biology
日期:2010-09-29
卷期号:: 221-229
被引量:11
标识
DOI:10.1007/978-1-60761-944-4_15
摘要
Alu PCR is a rapid and easy-to-perform “DNA fingerprinting” technique based on the simultaneous analysis of many genomic loci flanked by Alu repetitive elements, which allows the detection of genetic polymorphisms and mutations in human and primate genomes. In the protocol described in the present chapter, two fluorochrome-labelled primers complementary to Alu sequences are used to perform the PCR, and the amplification products are then analysed by capillary electrophoresis. The resulting complex electrophoretic pattern may show sample-to-sample variability due to insertion, deletion, or sequence change of Alu retrotransposons, or caused by length variation of the sequence interposed between two Alus.
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