Long-term exposure to antifouling biocide chlorothalonil modulates immunity and biochemical and antioxidant parameters in the blood of olive flounder

In this study, the potential effects of 30-day exposure to environmentally relevant concentrations of chlorothalonil (0, 5, 10, and 20 μg L-l) were tested in the blood of the economically important olive flounder, Paralichthys olivaceus, using biochemical endpoints. Significant decreases in the enzymatic activities of immunity markers, alternative complements, and lysozymes were detected in the fish exposed to 10 or 20 μg L-l of chlorothalonil at day 20 or 30. The total immunoglobulin content was lowered in response to 20 μg L-l chlorothalonil at day 10 and 20, even when later exposed to 5 μg L-l at day 30. Among the essential blood components, the cortisol level was increased in response to chlorothalonil throughout the study with a decrease in white blood cells, while no changes were observed in hemoglobin, red blood cells, total protein concentration, and glucose in all exposures. The enzymatic activities of the three hepatic toxicity markers, alanine transferases, aspartate transaminase, and alkaline phosphatase, increased by 10 and/or 20 μg L-l of chlorothalonil. Significant oxidative stress was induced by chlorothalonil in the fish exposed to 10 or 20 μg L-l of chlorothalonil, as revealed by increased malondialdehyde and fluctuating glutathione levels with increase in the enzymatic activities of antioxidant defense system, including catalase and superoxide dismutase, during exposure. Taken together, these results suggest that long-term exposure to environmentally relevant concentrations of chlorothalonil can affect susceptibility to pathogens through immunosuppression, hepatic toxicity, and oxidative stress in olive flounder. These results can contribute to the monitoring of aquatic environments and ecotoxicological research through the measurement of blood components against waterborne chlorothalonil.