生物
加巴能
睡眠(系统调用)
黑腹果蝇
生物钟
昼夜节律
神经科学
清醒
γ-氨基丁酸
基因敲除
细胞生物学
遗传学
抑制性突触后电位
受体
基因
操作系统
脑电图
计算机科学
作者
Ratna Chaturvedi,Tobias Stork,Chunyan Yuan,Marc R. Freeman,Patrick Emery
出处
期刊:Current Biology
[Elsevier]
日期:2022-03-01
卷期号:32 (9): 1895-1908.e5
被引量:3
标识
DOI:10.1016/j.cub.2022.02.066
摘要
Summary
A precise balance between sleep and wakefulness is essential to sustain a good quality of life and optimal brain function. GABA is known to play a key and conserved role in sleep control, and GABAergic tone should, therefore, be tightly controlled in sleep circuits. Here, we examined the role of the astrocytic GABA transporter (GAT) in sleep regulation using Drosophila melanogaster. We found that a hypomorphic gat mutation (gat33-1) increased sleep amount, decreased sleep latency, and increased sleep consolidation at night. Interestingly, sleep defects were suppressed when gat33-1 was combined with a mutation disrupting wide-awake (wake), a gene that regulates the cell-surface levels of the GABAA receptor resistance to dieldrin (RDL) in the wake-promoting large ventral lateral neurons (l-LNvs). Moreover, RNAi knockdown of rdl and its modulators dnlg4 and wake in these circadian neurons also suppressed gat33-1 sleep phenotypes. Brain immunohistochemistry showed that GAT-expressing astrocytes were located near RDL-positive l-LNv cell bodies and dendritic processes. We concluded that astrocytic GAT decreases GABAergic tone and RDL activation in arousal-promoting LNvs, thus determining proper sleep amount and quality in Drosophila.
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