琥珀酸
大肠杆菌
苹果酸
生物化学
苹果酸酶
醋酸
焊剂(冶金)
酶
化学
代谢通量分析
丙酮酸
代谢工程
拉伤
新陈代谢
生物
色谱法
有机化学
柠檬酸
脱氢酶
解剖
基因
作者
Soon Ho Hong,Sang Yup Lee
摘要
A pfl ldhA double mutant Escherichia coli strain NZN111 was used to produce succinic acid by overexpressing the E. coli malic enzyme. Escherichia coli strain NZN111 harboring pTrcML produced 6 and 8 g/L of succinic acid from 20 g/L of glucose in flask culture at 37 degrees C and 30 degrees C, respectively. When NZN111(pTrcML) was cultured at 30 degrees C with intermittent glucose feeding the final succinic acid concentration obtained was 9.5 g/L and the ratio of succinic acid to acetic acid was 13:1. This system could not be analyzed by conventional metabolic flux analysis techniques, since some pyruvate and succinic acid were accumulated intracellularly. Therefore, a new flux analysis method was proposed by introducing intracellular pyruvate and succinic acid pools. By this new method the concentrations of intracellular metabolites were successfully predicted and the differences between the measured and calculated reaction rates could be considerably reduced.
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