Study on process of total flavonoids in Chrysanthemun by Cellulase treatment

The technical factors of total flavonoids content by cellulase extraction from Chrysanthemum,it including:the addition of cellulase,cellulase treatment time,cellulase treatment temperature and pH.The extraction rate of optimized condition was determined by single factor experiment and orthogonal experiment. UV-Spectrophotometry was used for the determination of total flavonoids content.The optimum cellulase extraction process condition were:the addition of cellulase was 0.5%,cellulase treatment time was 2.5 h, cellulase treatment temperature was 55℃,cellulase treatment pH was 5.0.Under these conditions,the extraction rate of total flavonoids increase 19.2%contrast the control group.