Objective To investigate the chemical constituents of essential oil in Eupatorium adenophorum.Methods The chemical constituents of essential oil in Eupatorium adenophorum were analyzed by GC-MS with capillary column.The relative percentage contents of the constituents were determined by GC with area mormaliaing method.Results 84peaks were separated and 68 of them were identified.Conclusion The major chemical constituents of essential oil in Eupatorium adenophorum are terpenes.