Passive coupling of membrane tension and cell volume during active response of cells to osmosis

Significance Tension is the force-opposing stretch of lipid membranes. It controls cell functions involving membranes. Membranes rupture above a tension threshold, causing cell death if tension is not properly buffered. However, how cell membrane tension is quantitatively regulated is unknown because it is difficult to measure. Using a fluorescent membrane tension probe, we explored the coupling between membrane tension and cell volume changes during osmosis. This coupling is described by an equilibrium theory linking tension to folding and unfolding of the membrane. This coupling is nevertheless actively regulated by cell components such as the cytoskeleton, ion transporters, and mTOR pathways. Our results highlight that cell volume regulation and membrane tension homeostasis are independent from the regulation of their coupling.