体内
光热治疗
巨噬细胞
阿霉素
免疫系统
癌症研究
体外
材料科学
靶向给药
药物输送
医学
纳米技术
生物医学工程
化学
免疫学
化疗
生物
内科学
生物化学
生物技术
作者
Van Du Nguyen,Hyun-Ki Min,Ho Yong Kim,Jiwon Han,You Hee Choi,Chang‐Sei Kim,Jong-Oh Park,Eunpyo Choi
出处
期刊:ACS Nano
[American Chemical Society]
日期:2021-05-11
卷期号:15 (5): 8492-8506
被引量:44
标识
DOI:10.1021/acsnano.1c00114
摘要
Macrophages (MΦs) have the capability to sense chemotactic cues and to home tumors, therefore presenting a great approach to engineer these cells to deliver therapeutic agents to treat diseases. However, current cell-based drug delivery systems usually use commercial cell lines that may elicit an immune response when injected into a host animal. Furthermore, premature off-target drug release also remains an enormous challenge. Here, we isolated and differentiated MΦs from the spleens of BALB/c mice and developed dual-targeting MΦ-based microrobots, regulated by chemotaxis and an external magnetic field, and had a precise spatiotemporal controlled drug release at the tumor sites in response to the NIR laser irradiation. These microrobots were prepared by coloading citric acid (CA)-coated superparamagnetic nanoparticles (MNPs) and doxorubicin (DOX)-containing thermosensitive nanoliposomes (TSLPs) into the MΦs. CA-MNPs promoted a magnetic targeting function to the microrobots and also permitted photothermal heating in response to the NIR irradiation, triggering drug release from TSLPs. In vitro experiments showed that the microrobots effectively infiltrated tumors in 3D breast cancer tumor spheroids, particularly in the presence of the magnetic field, and effectively induced tumor cell death, further enhanced by the NIR laser irradiation. In vivo experiments confirmed that the application of the magnetic field and NIR laser could markedly inhibit the growth of tumors with a subtherapeutic dose of DOX and a single injection of the microrobots. In summary, the study proposes a strategy for the effective anticancer treatment using the developed microrobots.
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