清创术(牙科)
16S核糖体RNA
纳米孔测序
微生物学
拟杆菌
细菌
微生物培养
普雷沃菌属
生物
DNA测序
医学
外科
遗传学
基因
作者
Jangsup Moon,Narae Kim,Han Sang Lee,Soon‐Tae Lee,Keun‐Hwa Jung,Kyung‐Il Park,Sang Kun Lee,Dong‐Oh Lee,Dong Yeon Lee,Kon Chu
出处
期刊:Diabetes
[American Diabetes Association]
日期:2021-03-18
卷期号:70 (6): 1357-1371
被引量:29
摘要
Diabetic foot infections (DFIs) cause substantial morbidity and mortality. The mainstay of the treatment is empiric antibiotics and surgical debridement in severe cases. In this study, we performed nanopore 16S rDNA sequencing from the debridement specimens of DFIs. Fifty-four surgical debridement specimens obtained from 45 patients with medically intractable DFI were included. The 16S rDNA PCR was performed on each specimen, and Nanopore sequencing was performed for up to 3 h. The reads were aligned to the BLAST database, and the results were compared with conventional culture studies. The 16S sequencing results revealed that the majority of the DFIs (44 of 54, 81.5%) were polymicrobial infections. All bacteria isolated by conventional culture studies were detected by 16S sequencing. Several anaerobes (Prevotella, Finegoldia, Anaerococcus, Bacteroides) were commonly identified by 16S sequencing but were frequently missed by culture studies. In many cases, certain bacteria only revealed by the 16S sequencing were more abundant than the bacteria isolated by the culture studies. In conclusion, nanopore 16S sequencing was capable of pathogen identification in DFIs and has many advantages over conventional culture studies. Nanopore 16S sequencing enables a comprehensive understanding of the bacteria involved in DFIs.
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