钴胺素
化学
蛋氨酸合酶
共价键
生物化学
蛋氨酸
半胱氨酸
有机化学
氨基酸
酶
维生素B12
作者
Xin Chen,Jingyuan Xu,Nai-Kei Wong,Suyun Zhong,Mengquan Yang,Zhen Liu,Yan Lu,Weichao Li,Yiqing Zhou
标识
DOI:10.1021/acs.jafc.0c03301
摘要
Cobalamin-independent methionine synthases (MS) are zinc-binding methyltransferases that catalyze de novo methionine biosynthesis in higher plants, which are enzymes critically involved in seed germination and plant growth. Here, we report a highly selective sulfonyl fluoride-based probe for chemoproteomic profiling of MS enzymes in living systems of the model plant Arabidopsis thaliana, as implemented in in-gel-, mass spectrometry-, and imaging-based platforms. This probe holds promise for facilitating and accelerating fundamental research and industrial application of MS enzymes, particularly in the contexts of MS1/2-targeting herbicide screening and design.
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