波形蛋白
上皮-间质转换
免疫印迹
间充质干细胞
化学
癌症研究
污渍
细胞迁移
钙粘蛋白
细胞培养
免疫荧光
免疫组织化学
细胞
分子生物学
医学
过渡(遗传学)
病理
免疫学
生物
抗体
生物化学
基因
遗传学
作者
Wenjie Zhou,Jiao Chen,Yun Feng,Yanbo Fan,Qian Li,Jing Fu,Ping Zhang
出处
期刊:PubMed
日期:2016-07-01
卷期号:47 (4): 485-490
被引量:1
摘要
To explore the effects of the Smoothened (Smo) inhibitor PF-5274857 on cigarette smoke (CS)-induced epithelial-mesenchymal transition (EMT) and apply a new idea fororal squamous cell carcinoma (OSCC) treatment.Beas-2b cells were used to investigate the CS-induced EMT. Both pretreat and post-treat were performed in the study. In pretreat group, after being pretreated with 3 μmol/L PF-5274857 for 2 h, cells were cultured with CS for 8 d; In post-treat group, cells were treated by 3 μmol/L PF-5274857 for 4 d after CS cultrue. Western blot and immunofluorescence were applied to examine the EMT markers E-cadherin, vimentin, and smooth muscle actin α (α-SMA) in Beas-2b epithelial cells. The transwell culture system was used in migration assays.Pretreat Beas-2b cells with PF-5274857 for 2 h can prevent the CS-induced EMT for epithelial and mesenchymal markers, as well as migration capacity. Up regulated E-cadherin and down regulated vimentin and α-SMA were observed by Western blot. Furthermore Beas-2b cells induced by CS that underwent EMT showed increased E-cadherin and decreased vimentin and α-SMA after treatment with PF-5274857 for 4 d. Importantly, the elevated migration capacity level was also decreased.The Smo inhibitor PF-5274857 has both preventive effect and therapeutic potential against CS-induced EMT.
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