Human serum S100A12 level and severity of acute pancreatitis

Objective To evaluate the correlation between human serum S100A12 level and severity of acute pancreatitis (AP). Methods Serum S100A12 were tested in 64 AP patients in 24 hours after the onset. S100A12 levels was compared to the severity of AP , and the area under the curve (AUC)of the receiver operating characteristic curve(ROC) of serum S100A12 levels for estimating the severity of AP were made and compared with laboratory parameters and APACHE-Ⅱ, Ranson′s scoring system. Results S100A12 levels in early AP patients were higher than healthy controls(P patients scored 4-6>patients scored less than 4(P<0.01). S100A12 levels increased with organ failure (P<0.01). The AUC of S100A12 in 24 hours after onset for distinguishing MAP between MSAP and SAP was 0.80, cut-off point was 61.83 ng/ml, sensitivity was 70.01% and the specificity was 73.52%.Serum S100A12 levels were higher in SAP than in MSAP(P=0.01). The AUC of S100A12 in early AP for distinguishing between MSAP and SAP was 0.84, cut-off point was 285.32 ng/ml, the sensitivity was 76.94% and the specificity was 94.12%, Youden index was 71.00%, positive likelihood ratio was 13.00, negative likelihood ratio was 0.20, the diagnostic performance was better than APACHE-Ⅱ, Ranson and Marshall scoring systems and serum CRP concentration. Conclusions Human serum S100A12 levels elevated at early stage of AP. S100A12> 285.32 ng/ml represents high risk of SAP, which is more sensitive and accurate than APACHE-Ⅱ and Marshall scoring system. Key words: Pancreatitis, acute necrotizing; Calcium binding proteins; S100A12