Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum

Enzymatic lacto-N-biose transfer from lacto-N-biose 1,2-oxazoline to lactose by wild-type and glycosynthase variants of Bifidobacterium bifidum β-N-hexosaminidase yielded lacto-N-tetraose in a single-step, highly regioselective transformation.