微型加热器
微流控
混合器
炸薯条
材料科学
可靠性(半导体)
实验室晶片
制作
纳米技术
吞吐量
温度控制
重复性
计算机科学
功率(物理)
工程类
化学
色谱法
机械工程
医学
电信
替代医学
物理
病理
量子力学
无线
作者
H. Mashouf,Bahram Talebjedi,Nishat Tasnim,Maia Tan,Sahar Alousi,Sepideh Pakpour,Mina Hoorfar
标识
DOI:10.1016/j.cep.2023.109394
摘要
The production of microfluidic PCR devices within lab settings in a straightforward process and large numbers is limited due to complex methods of fabrication and high cost of development. In this study, we developed a unique disposable, low-cost, and easy-to-fabricate microfluidic PCR chip, comprised of a passive micromixer, reaction well, microheater, and a dynamic temperature control system. In addition to being compact, this device has the advantages of microfluidic PCR systems, such as low power and sample consumption, as well as being disposable and easy to fabricate. The disposable part of the chip is easy to fabricate and eliminates the run-to-run carryover contamination while its integration with a reusable heating system reduces the cost of producing a micro PCR device. The geometry of the micromixer and the pattern of microheater electrodes are analyzed through experiments to achieve comparable amplification to a conventional thermocycler. The temperature control system is designed to monitor the temperature within the reaction well with ±1 °C precision. Finally, the reliability and repeatability of the microfluidic device were verified by amplifying lambda (λ) DNA. The proposed device demonstrated high-quality amplification comparable to the conventional PCR and is an adaptable and low-cost on-chip PCR device for reliable molecular diagnostics.
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