Study on the Mechanism of Anti-Atopic Dermatitis by Herba Siegesbeckiae Based on Metabolomics

Background: Atopic dermatitis is a common inflammatory skin disease worldwide that is characterized by skin barrier dysfunction, itching, and a reduced quality of life. Objective: The research at hand aimed to delve into the anti-atopic dermatitis mechanism of Herba Siegesbeckiae, a traditional medicinal herb, using a metabolomic approach. Methods: The molecular mechanism by which Herba Siegesbeckiae acts against atopic dermatitis was investigated by establishing a mouse model of atopic dermatitis while conducting a metabolomics analysis on its metabolites. Results: Interleukin IL-13, IL-17A, IL-3, IL-31, IL-33, IL4, IL-5, TSLP, IgE, and histamine levels in serum, participating in inhibiting itching and regulating immunity signaling were found to be restored to varying degrees in AD treating with HS. A total of 31 differential metabolites were selected from metabolomics results, among which N-acetyl-L-alanine (VIP = 1.62), Nacetyl- L-methionine (VIP = 1.5), uracil (VIP = 1.47), and prostaglandin E2 (VIP = 1.4) play important roles in the anti-AD regulatory mechanisms of HS and can be used as biomarkers. In addition, the mechanisms of HS anti-AD have been shown to be associated with seven metabolic pathways, including β-alanine metabolism, glycerophospholipid metabolism, histidine metabolism, and so on. Conclusion: In conclusion, HS demonstrated properties that counteract Atopic Dermatitis by suppressing itchiness and boosting the immune system, subsequently controlling the concentrations of related metabolites.