Histone lactylation-mediated overexpression of RASD2 promotes endometriosis progression via upregulating the SUMOylation of CTPS1

子宫内膜异位症 组蛋白 机制(生物学) 癌症研究 相扑蛋白 组蛋白H2A 细胞生物学 EZH2型 组蛋白甲基转移酶 组蛋白甲基化 组蛋白密码 化学 生物 医学 内科学 基因 基因表达 生物化学 泛素 DNA甲基化 核小体 物理 量子力学
作者
Ziwei Wang,Yanhong Mao,Zihan Wang,Shuwei Li,Zhidan Hong,Rong Zhou,Shaoyuan Xu,Yao Xiong,Yuanzhen Zhang
出处
期刊:American Journal of Physiology-cell Physiology [American Physical Society]
标识
DOI:10.1152/ajpcell.00493.2024
摘要

Background: Histone lactylation is crucial in a variety of physiopathological processes, however, the function and mechanism of histone lactylation in endometriosis remain poorly understood. Therefore, the objective of this investigation was to illuminate the function and mechanism of histone lactylation in endometriosis. Methods: Immunohistochemistry was used to investigate the expression of histone lactylation. Cell Counting Kit-8 assay (CCK8), Transwell assay and endometriosis mouse models were used to investigate the effects of histone lactylation in vitro and in vivo. Transcriptomics and Immunoprecipitation-Mass Spectrometry (IP-MS), Western Blot, Co-Immunoprecipitation (Co-IP), quantitative reverse transcription polymerase chain reaction (qRT-PCR) and chromatin immunoprecipitation-qPCR (ChIP-qPCR) were used to explore the intrinsic mechanisms. Results: In this study, we found that histone lactylation was upregulated in endometriosis and could promote endometriosis progression both in vivo and in vitro. Mechanistically, histone lactylation H3K18la promoted the transcription of Ras homolog enriched in striatum (RASD2), and RASD2, in turn, increased the stability of CTP synthase 1 (CTPS1) by promoting the SUMOylation and inhibiting the ubiquitination of CTPS1, thereby promoting endometriosis progression. Conclusion: Overall, our findings indicated that histone lactylation could promote the progression of endometriosis through the RASD2/CTPS1 axis. This investigation uncovered a novel mechanism and identified prospective targets for endometriosis diagnosis and therapy.
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