Dual role of endophytic entomopathogenic fungi: induce plant growth and control tomato leafminer Phthorimaea absoluta

Entomopathogenic fungi (EPF) are multifunctional microorganisms acting not only as biopesticides against insect pests but also as endophytes which regulate plant growth. The tomato leafminer, Phthorimaea absoluta (Tuta absoluta) is a devastating invasive pest of tomatoes worldwide. However, effective alternatives are needed for a sustainable management of this invasive pest. In this study, the functional effects of five EPF isolates Metarhizium flavoviride, M. anisopliae, M. rileyi, Cordyceps fumosorosea and Beauveria bassiana were evaluated on tomato growth promotion and pest protection against P. absoluta.When directly sprayed with conidia, P. absoluta larvae showed high cumulative mortality of 100% to M. anisopliae under 1 × 108 conidia/mL, whereas M. flavoviride, B. bassiana, C. fumosorosea and M. rileyi caused cumulative mortality of 92.65%, 92.62%, 92.16% and 68.95%, respectively. Moreover, all five EPF isolates can successfully colonize tomato plants, whilst the colonization rate for each EPF depends on the inoculation method used. The most efficient inoculation method for M. flavoviride and M. rileyi was root dipping, for M. anisopliae and C. fumosorosea it was coating seed, and for B. bassiana it was foliage spraying. The highest plant colonization was obtained by M. flavoviride. Meanwhile, all these isolates promoted tomato plant growth upon inoculation. Furthermore, endophytic colonization of plants by the five EPF negatively affected the performance of P. absoluta, among them M. anisopliae and C. fumosorosea showed strong negative effects on the performance of P. absoluta.Our results highlight the potential of incorporating entomopathogenic fungi as endophytes in integrated pest management practices to protect tomatoes against P. absoluta. © 2023 Society of Chemical Industry.