白腐真菌
生物转化
黄孢菌
代谢物
化学
木质素过氧化物酶
锰过氧化物酶
酶
生物化学
毒性
羟基化
微生物学
过氧化物酶
生物
有机化学
作者
Shiye Zhu,Anwei Chen,Youzheng Chai,Ruoyu Cao,Jianhua Zeng,Ma Bai,Liang Peng,Jihai Shao,Xiang Wang
标识
DOI:10.1016/j.cej.2023.144798
摘要
As one of the widely used neonicotinoid insecticide, imidacloprid (IMI) poses serious threat on many kinds of non-target species. The acceleration of its removal from the contaminated environment is therefore an urgent task. Herein, the transformation of IMI by white-rot fungus Phanerochaete chrysosporium was examined, and the reaction mechanism underlying enzymes-mediated IMI biotransformation was elucidated. In addition, transformed products, pathways, and toxicity to Escherichia coli and Microcystis aeruginosa or the growth of rice seeds were investigated. P. chrysosporium could efficiently transform 93.5% of IMI for 6 days primarily through biotransformation, in which extracellular manganese peroxidase and lignin peroxidase substantially responded to transform IMI. IMI mainly underwent nitrate reduction, hydroxylation, and de-chlorination. A less neurologically toxic major metabolite of IMI, 6-chloronicotinic acid, was detected. Molecular dynamics simulation demonstrated that IMI stably bonded to active pocket of extracellular enzymes. This study reveals the key metabolic mechanism and fate of IMI in environment, and highlights the potential of fungi for IMI transformation.
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