低温电子显微
单粒子分析
分辨率(逻辑)
纳米技术
显微镜
电子显微镜
高分辨率
粒子(生态学)
计算机科学
生物系统
物理
材料科学
人工智能
生物
核磁共振
光学
遥感
生态学
气溶胶
气象学
地质学
作者
Ashwin Chari,Holger Stark
标识
DOI:10.1146/annurev-biophys-111622-091300
摘要
Single particle cryo-electron microscopy (cryo-EM) has matured into a robust method for the determination of biological macromolecule structures in the past decade, complementing X-ray crystallography and nuclear magnetic resonance. Constant methodological improvements in both cryo-EM hardware and image processing software continue to contribute to an exponential growth in the number of structures solved annually. In this review, we provide a historical view of the many steps that were required to make cryo-EM a successful method for the determination of high-resolution protein complex structures. We further discuss aspects of cryo-EM methodology that are the greatest pitfalls challenging successful structure determination to date. Lastly, we highlight and propose potential future developments that would improve the method even further in the near future.
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