Dual-Mode Virus Detection: Combining Electrochemical and Fluorescence Modalities for Enhanced Sensitivity and Reliability

This study introduces a dual-mode biosensor specifically designed for the quantitative detection of viruses in rapid analysis. The biosensor is unique in its use of both optical (fluorescence) and electrochemical (impedance) detection methods using the same nanocomposites, providing a dual confirmation system for virus (norovirus-like particles) quantification. The system is based on using two antibody-conjugated nanocomposites: CdSeS quantum dots and Au-N,S-GQD nanocomposites. For optical detection, the principle relies on the fluorescence quenching of CdSeS by Au-N,S-GQD in a sandwich structure with the target. Conversely, electrochemical detection is based on the change in impedance caused by the formation of the same sandwich structure. The biosensor demonstrated exceptional sensitivity, capable of detecting norovirus at concentrations of as low as femtomolar in the electrochemical method and picomolar in the optical method. In the dual-responsive concentration range from 10-13 to 10-10 M, the sensor is highly sensitive in both methods, creating significant changes in fluorescence intensity and impedance in the presence of virus. Furthermore, the biosensor exhibits a high degree of specificity, with a negligible response to nontarget proteins, even within complex test solutions. This work represents a significant advancement in the field of biosensor technology, offering a fast, accurate, and reliable method for diagnosing viral infections and diseases.