亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Methionine-producing tumor micro(be) environment fuels growth of solid tumors

蛋氨酸 生物 代谢组 微生物群 营养不良 细胞生长 细胞周期 癌症研究 微生物学 生物化学 氨基酸 细胞 大肠杆菌 代谢组学 遗传学 生物信息学 基因
作者
Alexis A. Vega,Erin A. Marshall,Avery J. C. Noonan,Fernando Sergio Leitão Filho,Julia Yang,Greg L. Stewart,Fraser Johnson,Emily A. Vucic,Michelle E. Pewarchuk,Parag P. Shah,Brian F. Clem,Corey Nislow,Stephen Lam,William W. Lockwood,Steven J. Hallam,Janice M. Leung,Levi J. Beverly,Wan L. Lam
出处
期刊:Cellular oncology [Springer Nature]
卷期号:46 (6): 1659-1673 被引量:4
标识
DOI:10.1007/s13402-023-00832-7
摘要

Abstract Background Recent studies have uncovered the near-ubiquitous presence of microbes in solid tumors of diverse origins. Previous literature has shown the impact of specific bacterial species on the progression of cancer. We propose that local microbial dysbiosis enables certain cancer phenotypes through provisioning of essential metabolites directly to tumor cells. Methods 16S rDNA sequencing of 75 patient lung samples revealed the lung tumor microbiome specifically enriched for bacteria capable of producing methionine. Wild-type (WT) and methionine auxotrophic (metA mutant) E. coli cells were used to condition cell culture media and the proliferation of lung adenocarcinoma (LUAD) cells were measured using SYTO60 staining. Further, colony forming assay, Annexin V Staining, BrdU, AlamarBlue, western blot, qPCR, LINE microarray and subcutaneous injection with methionine modulated feed were used to analyze cellular proliferation, cell-cycle, cell death, methylation potential, and xenograft formation under methionine restriction. Moreover, C 14 -labeled glucose was used to illustrate the interplay between tumor cells and bacteria. Results/Discussion Our results show bacteria found locally within the tumor microenvironment are enriched for methionine synthetic pathways, while having reduced S-adenosylmethionine metabolizing pathways. As methionine is one of nine essential amino acids that mammals are unable to synthesize de novo, we investigated a potentially novel function for the microbiome, supplying essential nutrients, such as methionine, to cancer cells. We demonstrate that LUAD cells can utilize methionine generated by bacteria to rescue phenotypes that would otherwise be inhibited due to nutrient restriction. In addition to this, with WT and metA mutant E. coli , we saw a selective advantage for bacteria with an intact methionine synthetic pathway to survive under the conditions induced by LUAD cells. These results would suggest that there is a potential bi-directional cross-talk between the local microbiome and adjacent tumor cells. In this study, we focused on methionine as one of the critical molecules, but we also hypothesize that additional bacterial metabolites may also be utilized by LUAD. Indeed, our radiolabeling data suggest that other biomolecules are shared between cancer cells and bacteria. Thus, modulating the local microbiome may have an indirect effect on tumor development, progression, and metastasis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
banana完成签到 ,获得积分10
2秒前
5秒前
提速狗完成签到,获得积分10
43秒前
50秒前
52秒前
1分钟前
白派派主发布了新的文献求助10
1分钟前
w1x2123完成签到,获得积分10
1分钟前
万能图书馆应助hanzhang采纳,获得10
1分钟前
1分钟前
1分钟前
1分钟前
messi发布了新的文献求助80
1分钟前
hanzhang发布了新的文献求助10
1分钟前
1分钟前
1分钟前
白云朵儿发布了新的文献求助10
1分钟前
棋朵朵完成签到,获得积分10
1分钟前
棋朵朵发布了新的文献求助10
2分钟前
冷佳蓥完成签到,获得积分20
2分钟前
Orange应助hanzhang采纳,获得10
2分钟前
6w6完成签到 ,获得积分10
3分钟前
白派派主完成签到,获得积分20
3分钟前
3分钟前
眼睛大半烟完成签到 ,获得积分10
3分钟前
liu576454693发布了新的文献求助10
3分钟前
科研通AI2S应助liu576454693采纳,获得10
3分钟前
聪明的老鼠完成签到,获得积分10
4分钟前
rocky15应助尊敬曼岚采纳,获得10
4分钟前
白派派主发布了新的文献求助10
4分钟前
4分钟前
DAYUEZI完成签到 ,获得积分10
4分钟前
一大只北极熊完成签到,获得积分10
4分钟前
messi发布了新的文献求助10
4分钟前
4分钟前
Ava应助messi采纳,获得10
4分钟前
Likj完成签到,获得积分10
5分钟前
5分钟前
hanzhang发布了新的文献求助10
5分钟前
echoMe完成签到 ,获得积分10
6分钟前
高分求助中
Un calendrier babylonien des travaux, des signes et des mois: Séries iqqur îpuš 1036
Sustainable Land Management: Strategies to Cope with the Marginalisation of Agriculture 1000
Corrosion and Oxygen Control 600
Heterocyclic Stilbene and Bibenzyl Derivatives in Liverworts: Distribution, Structures, Total Synthesis and Biological Activity 500
重庆市新能源汽车产业大数据招商指南(两链两图两池两库两平台两清单两报告) 400
Division and square root. Digit-recurrence algorithms and implementations 400
行動データの計算論モデリング 強化学習モデルを例として 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2545500
求助须知:如何正确求助?哪些是违规求助? 2175642
关于积分的说明 5600159
捐赠科研通 1896337
什么是DOI,文献DOI怎么找? 946234
版权声明 565355
科研通“疑难数据库(出版商)”最低求助积分说明 503557