生物
核糖核酸
病毒
细胞周期
阿尔戈瑙特
病毒生命周期
小RNA
计算生物学
基因表达调控
RNA干扰
细胞生物学
基因
遗传学
作者
Sarah Ressel,Sujai Kumar,José Roberto Bermúdez‐Barrientos,Katrina Gordon,Julia Lane,Wei Jin,Cei Abreu‐Goodger,Jürgen Schwarze,Amy H. Buck
摘要
Abstract microRNAs (miRNAs) regulate nearly all physiological processes but our understanding of exactly how they function remains incomplete, particularly in the context of viral infections. Here, we adapt a biochemical method (CLEAR-CLIP) and analysis pipeline to identify targets of miRNAs in lung cells infected with Respiratory syncytial virus (RSV). We show that RSV binds directly to miR-26 and miR-27 through seed pairing and demonstrate that these miRNAs target distinct gene networks associated with cell cycle and metabolism (miR-27) and antiviral immunity (miR-26). Many of the targets are de-repressed upon infection and we show that the miR-27 targets most sensitive to miRNA inhibition are those associated with cell cycle. Finally, we demonstrate that high confidence chimeras map to long noncoding RNAs (lncRNAs) and pseudogenes in transcriptional regulatory regions. We validate that a proportion of miR-27 and Argonaute 2 (AGO2) is nuclear and identify a long non-coding RNA (lncRNA) as a miR-27 target that is linked to transcriptional regulation of nearby genes. This work expands the target networks of miR-26 and miR-27 to include direct interactions with RSV and lncRNAs and implicate these miRNAs in regulation of key genes that impact the viral life cycle associated with cell cycle, metabolism, and antiviral immunity.
科研通智能强力驱动
Strongly Powered by AbleSci AI