细胞毒性T细胞
医学
细胞因子诱导的杀伤细胞
结直肠癌
颗粒酶B
免疫疗法
免疫学
细胞因子
癌症研究
外周血单个核细胞
颗粒酶
穿孔素
癌症
免疫系统
T细胞
生物
体外
CD8型
内科学
CD3型
生物化学
作者
Celine Man Ying Li,Yoko Tomita,Bimala Dhakal,Teresa Tin,Runhao Li,Josephine A. Wright,Laura Vrbanac,Susan L. Woods,Paul A. Drew,Timothy Price,Eric Smith,Guy J. Maddern,Kevin Fenix
标识
DOI:10.1007/s00262-023-03591-4
摘要
Abstract Colorectal cancer (CRC) is the second leading cause of cancer-related death worldwide. Cytokine-induced killer (CIK) cells are an adoptive immunotherapy reported to have strong anti-tumour activity across a range of cancers. They are a heterogeneous mix of lymphoid cells generated by culturing human peripheral blood mononuclear cells with cytokines and monoclonal antibodies in vitro. In this study, we investigated the yield and function of CIK cells generated from patients with CRC liver metastases. We first showed that CIK cells generated in serum free medium X-VIVO 15 were comparable to those from RPMI medium with 10% FBS in terms of the number and percentages of the main subsets of cells in the CIK culture, and the intracellular levels of granzyme B and perforin, and the pro-inflammatory cytokines IL-2, IFN-γ and TNF-α. The CIK cells were cytotoxic to CRC cell lines grown in 2D cultures or as spheroids, and against autologous patient-derived tumour organoids. Donor attributes such as age, sex, or prior chemotherapy exposure had no significant impact on CIK cell numbers or function. These results suggest that functional CIK cells can be generated from patients with CRC liver metastatic disease, and support further investigations into the therapeutic application of autologous CIK cells in the management of patients with CRC liver metastases.
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