A resorufin-based fluorescent probe for hydrazine detection and its application in environmental analysis and bioimaging

Hydrazine (N₂H₄) is an important industrial raw material that has been widely used in industrial production and agricultural interventions, but its widespread application also inevitably causes environmental pollution. In this study, based on resorufin, we constructed a novel "turn-on" fluorescent probe RFT for the selective detection of hydrazine under complex environmental conditions and in vivo. The probe RFT exhibited excellent stability and selectivity towards the detection of hydrazine with a low detection limit of 260 nM. In addition, RFT was successfully applied to the detection of hydrazine in environmental water samples and living cells. Most importantly, RFT could not only detect the exogenous hydrazine in zebrafish and mice, but also image and visualize the up-regulation of endogenous hydrazine induced by isoniazid in zebrafish.