化学
脂多糖
透明质酸
软骨细胞
细胞凋亡
阿格里坎
炎症
免疫印迹
自噬
生物化学
细胞生长
分子生物学
药理学
细胞生物学
骨关节炎
生物
免疫学
医学
基因
病理
关节软骨
遗传学
替代医学
体外
作者
Hesuyuan Huang,Xuyang Ding,Dan Xing,Jianjing Lin,Zhongtang Li,Jianhao Lin
出处
期刊:Molecules
[MDPI AG]
日期:2022-08-31
卷期号:27 (17): 5619-5619
被引量:1
标识
DOI:10.3390/molecules27175619
摘要
High molecular weight hyaluronic acids (HMW-HAs) have been used for the palliative treatment of osteoarthritis (OA) for decades, but the pharmacological activity of HA fragments has not been fully explored due to the limited availability of structurally defined HA fragments. In this study, we synthesized a series glycosides of oligosaccharides of HA (o-HAs), hereinafter collectively referred to as o-HA derivatives. Their effects on OA progression were examined in a chondrocyte inflammatory model established by the lipopolysaccharide (LPS)-challenged ATDC5 cells. Cell Counting Kit-8 (CCK-8) assays and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) showed that o-HA derivatives (≤100 μg/mL) exhibited no cytotoxicity and pro-inflammatory effects. We found that the o-HA and o-HA derivatives alleviated LPS-induced inflammation, apoptosis, autophagy and proliferation-inhibition of ATDC5 cells, similar to the activities of HMW-HAs. Moreover, Western blot analysis showed that different HA derivatives selectively reversed the effects of LPS on the expression of extracellular matrix (ECM)-related proteins (MMP13, COL2A1 and Aggrecan) in ATDC5 cells. Our study suggested that o-HA derivatives may alleviate LPS-induced chondrocyte injury by reducing the inflammatory response, maintaining cell proliferation, inhibiting apoptosis and autophagy, and decreasing ECM degradation, supporting a potential oligosaccharides-mediated therapy for OA.
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