Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA

Stable cell lines of Chinese hamster ovary (CHO) cells are the predominant source of commercial ­biopharmaceutical proteins. Because making suitable CHO cell lines is time-consuming and costly, ­preliminary experiments with transient expression are usually performed to optimize as many protein ­production parameters as possible. Here, we describe protocols for optimizing expression in transient expression experiments and isolating stable CHO cell lines using two types of self-made reagents, namely, lipoplexes and polyplexes.