内皮抑素
血管生成
背景(考古学)
体内
化学
基质凝胶
体外
脐静脉
内皮干细胞
药理学
药物输送
细胞生物学
癌症研究
绒毛尿囊膜
新生血管
离体
生物
医学
生物化学
生物技术
有机化学
古生物学
作者
Dag K. Skovseth,Axel M. Küchler,Guttorm Haraldsen
出处
期刊:Humana Press eBooks
[Humana Press]
日期:2006-12-16
卷期号:: 253-268
被引量:7
标识
DOI:10.1385/1-59745-165-7:253
摘要
SummaryThe future ability to manipulate the growth of new blood vessels (angiogenesis) holds great promise for treating ischemic disease and cancer. Several models of human in vivo angiogenesis have been described, but they seem to depend on transgenic support and have not been validated in a therapeutic context. Here, we describe an in vivo assay that mimics human angiogenesis in which native human umbilical vein-derived endothelial cells are suspended in a liquid laminin/collagen gel (Matrigel), injected into immunodeficient mice, and develop into mature, functional vessels that vascularize the Matrigel plug in the course of 30 d. Moreover, we demonstrate how to target this process therapeutically by sustained delivery of the angiogenesis inhibitor endostatin from subcutaneously implanted microosmotic pumps.
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