色谱法
串联质谱法
质谱法
蛋白质组学
化学
液相色谱-质谱法
肽
自下而上蛋白质组学
电喷雾
蛋白质质谱法
生物化学
基因
作者
Marco Gaspari,Giovanni Cuda
出处
期刊:Methods in molecular biology
日期:2011-01-01
卷期号:: 115-126
被引量:54
标识
DOI:10.1007/978-1-61779-319-6_9
摘要
Nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) has become an essential tool in the field of proteomics. In fact, its sensitivity has advantages over conventional LC-MS/MS that allow the analysis of peptide mixtures in sample-limited situations (e.g., proteolytically digested proteins isolated by two-dimensional gel electrophoresis). Technical challenges, associated with low flow rates of the chromatographic separation, make this technology still difficult to run routinely. Here, we describe a nano LC-MS/MS setup that allows several weeks of continuous operation for the analysis of peptides derived by enzymatic digestion of either purified proteins or moderately complex protein mixtures.
科研通智能强力驱动
Strongly Powered by AbleSci AI