槲皮素
化学
杨梅素
IC50型
生物信息学
传统医学
色谱法
类黄酮
高效液相色谱法
体外
生物化学
山奈酚
抗氧化剂
医学
基因
作者
Songjie Li,Ruimin Wang,Xiaoping Hu,Congfa Li,Lu Wang
出处
期刊:Food Chemistry
[Elsevier]
日期:2022-03-01
卷期号:373: 131528-131528
被引量:31
标识
DOI:10.1016/j.foodchem.2021.131528
摘要
Cerasus humilis(Bge.) Sok. leaf-tea (CLT) has a potential anti-α-glucosidase effect. However, its anti-α-glucosidase functional compositions remain unclear. Results showed that 70% methanol extract of CLT (IC50 = 36.57 μg/mL) with the highest total phenolic/flavonoid contents exhibited significantly higher α-glucosidase inhibitory activity (α-GIA) than acarbose (IC50 = 189.57 μg/mL). Additionally, phenolic constituents of the CLT extract were analyzed for the first time in this work. Ten major potential α-glucosidase inhibitors (α-GIs) with high bio-affinity degree in the CLT extract were recognized using a bio-affinity ultra-filtration and HPLC-ESI-qTOF-MS/MS method. In vitro α-GIA assay confirmed that myricetin (IC50 = 36.17 μg/mL), avicularin (IC50 = 69.84 μg/mL), quercitrin, isoquercitrin, prunin and guajavarin were responsible for the α-GIA of the CLT extract. More importantly, the interaction mechanism between α-GIs and α-glucosidase was investigated via in silico analysis. This study provides a high-throughput screening platform for identification of the potential α-GIs from natural products.
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