RNA干扰
黄萎病
生物
大丽花黄萎病
真菌
RNA沉默
植物病害
核糖核酸
植物
生物技术
基因
遗传学
作者
Tao Zhang,Fei Wang,Hui‐Shan Guo,Yipeng Jin
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 243-252
标识
DOI:10.1007/978-1-0716-1875-2_16
摘要
Trans-kingdom RNA interference (RNAi) has been reported in several plant-fungal pathosystems. Our recent works have demonstrated natural RNAi transmission from cotton plants into Verticillium dahliae, a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues, and successful application of trans-kingdom RNAi in cotton plants to confer Verticillium wilt disease resistance. Here, we provide a detailed protocol of cotton infection with V. dahliae, fungal hyphae recovery from infected cotton stems, and transmitted small RNA detection developed from our previous studies for trans-kingdom RNAi assays.
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