生殖系
胚胎干细胞
生物
分子生物学
聚合酶链反应
突变
干细胞
遗传学
分离(微生物学)
基因
生物信息学
作者
Virginia E. Papaioannou,Richard R. Behringer
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2023-11-06
卷期号:2024 (1): pdb.prot108038-pdb.prot108038
被引量:1
标识
DOI:10.1101/pdb.prot108038
摘要
This is a simple procedure to isolate XO subclones from XY murine embryonic stem cells in situations that require transmission of a mutation through the female germline-for example, if the mutation adversely affects spermatogenesis. XY cells are plated at clonal density, and resulting colonies are genotyped by polymerase chain reaction for a Y-specific probe to identify clones that have spontaneously lost the Y chromosome.
科研通智能强力驱动
Strongly Powered by AbleSci AI