污水
回声病毒
肠道病毒
血清型
柯萨奇病毒
生物
遗传多样性
兽医学
放大器
人口
生态学
病毒学
遗传学
病毒
聚合酶链反应
基因
环境科学
环境卫生
环境工程
医学
作者
Rongrong Huang,Xiaojuan Lin,Peng Chen,Feng Jiang,Yao Liu,Suting Wang,Meng Chen,Ke Song,Zexin Tao,Aiqiang Xu
标识
DOI:10.1093/jambio/lxad028
摘要
Next generation sequencing (NGS) technology has been widely used in monitoring and identifying microbiomes in sewage. We aimed to evaluate the ability of NGS analysis in directly detecting enterovirus (EV) in sewage and to understand the diversity of EVs circulated in the residents in Weishan Lake region.Fourteen sewage samples were collected in Jining, Shandong Province, China from 2018 to 2019 and were parallelly investigated by the P1 amplicon-based NGS method and cell culture method. The results showed that 20 different serotypes belonging to species Enterovirus A (EV-A) (n = 5), EV-B (n = 13), and EV-C (n = 2) were identified by NGS in the sewage concentrates, which exceeded the number of types detected by cell culture method (n = 9). Echovirus 11 (E11), Coxsackievirus (CV) B5 and CVA9 were the most detected types in those sewage concentrates. Phylogenetic analysis revealed that E11 sequences obtained in this study belonged to genogroup D5 and had close genetic relationship with clinical sequences.Various EV serotypes circulated in populations near Weishan Lake. The combination of NGS technology into environmental surveillance will greatly contribute to improving our knowledge about EV circulation patterns in the population.
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