N6-甲基腺苷
拟南芥
RNA干扰
生物
翻译效率
翻译(生物学)
平动调节
基因表达
非翻译区
基因
细胞生物学
信使核糖核酸
拟南芥
核糖核酸
遗传学
突变体
甲基化
甲基转移酶
作者
Shuai Wang,Haiyan Wang,Zhihui Xu,Shasha Jiang,Yucheng Shi,Hairong Xie,Shu Wang,Jian Hua,Yufeng Wu
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2023-02-22
卷期号:192 (2): 1466-1482
被引量:12
标识
DOI:10.1093/plphys/kiad112
摘要
N 6-methyladenosine (m6A), the most prevalent mRNA modification in eukaryotes, is an emerging player of gene regulation at transcriptional and translational levels. Here, we explored the role of m6A modification in response to low temperature in Arabidopsis (Arabidopsis thaliana). Knocking down mRNA adenosine methylase A (MTA), a key component of the modification complex, by RNA interference (RNAi) led to drastically reduced growth at low temperature, indicating a critical role of m6A modification in the chilling response. Cold treatment reduced the overall m6A modification level of mRNAs especially at the 3' untranslated region. Joint analysis of the m6A methylome, transcriptome and translatome of the wild type (WT) and the MTA RNAi line revealed that m6A-containing mRNAs generally had higher abundance and translation efficiency than non-m6A-containing mRNAs under normal and low temperatures. In addition, reduction of m6A modification by MTA RNAi only moderately altered the gene expression response to low temperature but led to dysregulation of translation efficiencies of one third of the genes of the genome in response to cold. We tested the function of the m6A-modified cold-responsive gene ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE 1 (DGAT1) whose translation efficiency but not transcript level was reduced in the chilling-susceptible MTA RNAi plant. The dgat1 loss-of-function mutant exhibited reduced growth under cold stress. These results reveal a critical role of m6A modification in regulating growth under low temperature and suggest an involvement of translational control in chilling responses in Arabidopsis.
科研通智能强力驱动
Strongly Powered by AbleSci AI