Thin-Film Hydration Followed by Extrusion Method for Liposome Preparation

One of the simplest ways to prepare liposomesLiposomes in a research laboratory is the thin-film hydrationThin-film hydration method followed by extrusionExtrusion. This method involves making a thin lipidLipids film in a round-bottom flask by the removal of organic solvent. Upon the addition and agitation of the dispersion medium, heterogeneous liposomesLiposomes are formed. Finally, after extrusionExtrusion through polycarbonate membranesMembranes, homogeneous small liposomesLiposomes are obtained.