舍瓦内拉
化学
氧化还原
微生物燃料电池
纳米颗粒
生物传感器
纳米技术
电子转移
细菌
电极
遗传学
生物
阳极
物理化学
有机化学
材料科学
作者
Kai Yang,Zijie Lu,Tianyu Zhu,Jing-Xian Wang,Fu-Qiao Yang,Syed Zaghum Abbas,Jun Zhou,Zhugen Yang,Jianli Mi,Sai Kishore Ravi,Yang‐Chun Yong
标识
DOI:10.1016/j.aca.2023.341046
摘要
Whole-cell biosensors are an important class of analytical tools that offer the advantages of low cost, facile operation, and unique reproduction/regeneration ability. However, it has always been quite challenging to expand the sensing spectrum of the host. Here, a new approach to extend the cell sensing spectrum with biomineralized nanoparticles is developed. The nano-biohybrid design comprise biomineralized FeS nanoparticles firmly anchored onto the bacterium, Shewanella oneidensis MR-1, wherein the nanoparticles are wired to the cellular electron transfer machinery (MtrCAB/OmcA) of the bacterium, forming an artificial cyborgian redox machinery consisting of FeS-MtrCAB/OmcA-FccA. Strikingly, with this cyborgian redox machinery, the sensing spectrum of FeS hybridized S. oneidensis MR-1 cell is successfully expanded to enable whole-cell electrochemical detection of Vitamin B12, while an unhybridized native cell is incapable of sensing. This proof-of-concept nano-biohybrid design offers a new perspective on manipulating the microbial toolkit for an expanded sensing spectrum in whole-cell biosensors.
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