High performance liquid chromatographic separation of platinum (II), gold (III), vanadium (IV), vanadium (V), molybdenum (VI) and analysis of cis-platin as platinum (II) in cis-plasol injection, urine, and blood serum using pyridoxal-4-phenyl-3-thiosemicarbazone as complexing reagent

A high performance liquid chromatographic (HPLC) method has been proposed for separation of Pt (II), Au (III), V (IV)/V (V), Mo (VI) and analysis of cis-platin on the basis of pre column derivatization using pyridoxal-4-phenyl-3-thiosemicarbazone (PDPTSC) as complexing reagent. The complexes were extracted in trichloromethane or n-butanol and were separated from Kromacil100, C-18, 10 µm (250 cm × 4.6 mm, i.d) column with a ternary mixture of acetonitrile:water:methanol (20:30:50 V/V/V) with a flow rate of 1 mL.min−1 and ultra-violet detection was at 320 nm. The linear calibration was achieved with 0.5–5 µg.mL−1 and detection limit of 10 ng.mL−1 platinum (II). The determination of cis-platin (cis-plasol) injection by HPLC method showed RSD 2.41%. The developed method was employed for the analysis of cis-platin from urine and blood serum of cancer patients after chemotherapy and platinum contents were found 47.0–87.0 ng.mL−1 and 77.4–424 ng.mL−1 with RSD within 0.824–3.42% for urine and 0.421–3.84% for serum. The recovery of platinum (II) was found as 98.1% with RSD 1.99%.