内质网
线粒体
三磷酸腺苷
农奴
细胞生物学
细胞器
生物
ATP酶
生物化学
酶
作者
Xiao Li Jiang,Jia Hui Liu,Yan Ting Que,Yu Mei Que,Ping Hu,Cheng Huang,Xiaoyong Tong
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2019-12-19
卷期号:6 (3): 1727-1734
被引量:7
标识
DOI:10.1021/acsbiomaterials.9b01395
摘要
Mitochondria are critical organelles in eukaryotes that efficiently generate adenosine 5′-triphosphate (ATP) for various biological activities, and any defect in the process of ATP synthesis may lead to mitochondrial dysfunction and directly link to a variety of medical disorders. Monitoring the ATP variations in cells is key for innovative early diagnosis of mitochondrial diseases. Herein, multifunctional single-layered graphene quantum dots (s-GQDs) with bright green emission were constructed, which exhibit strong binding affinity for ATP and good mitochondria targeting ability. Using the proposed s-GQDs, we successfully discriminated the primary smooth muscle cells isolated from the transgenic mouse (heterozygote sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) 2 C674S knock-in mouse) with mitochondrial disorders or their littermate controls, indicating s-GQDs as promising probes for the study of cell metabolism and mitochondrial malfunction-related diseases, and targeting endoplasmic reticulum stress is an effective way to modulate metabolic pathways relevant to SERCA 2 inactivity mitochondrial dysfunction.
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