嵌合抗原受体
受体
抗原
细胞生物学
合理设计
生物
抗原提呈细胞
计算生物学
癌症免疫疗法
免疫疗法
化学
免疫学
T细胞
遗传学
生物化学
免疫系统
作者
ZeNan Chang,Andrew J. Hou,Yvonne Y. Chen
出处
期刊:Nature Protocols
[Springer Nature]
日期:2020-02-26
卷期号:15 (4): 1507-1524
被引量:18
标识
DOI:10.1038/s41596-020-0294-8
摘要
The expression of synthetic receptors in primary T cells enables the programming of user-defined responses when designing T-cell therapies. Chimeric antigen receptors (CARs) are synthetic receptors that have demonstrated efficacy in cancer therapy by targeting immobilized antigens on the surface of malignant cells. Recently, we showed they can also rewire T-cell responses to soluble ligands. In contrast to other synthetic receptors, CARs are not only readily engineered by rational design, but also clinically translatable, with robust function in primary human T cells. This protocol discusses design principles for CARs responsive to soluble ligands and delineates steps for producing T cells expressing synthetic receptors. Functional assays for quantifying the ability of CAR T cells to sense and respond to soluble ligands are also presented. This protocol provides a framework for proficient immune-cell researchers to test novel T-cell therapies targeting soluble ligands in <2 weeks. This protocol discusses design principles for CARs responsive to soluble ligands and delineates steps for producing T cells expressing synthetic receptors. Functional assays for quantifying the ability of CAR T cells to sense and respond to soluble ligands are also presented.
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