雅罗维亚
柠檬酸
柠檬酸合酶
生物化学
生物合成
ATP柠檬酸裂解酶
酵母
化学
ATP合酶
丙酸盐
突变体
拉伤
基因
生物
酶
解剖
作者
Piotr Hapeta,Magdalena Rakicka‐Pustułka,Piotr Juszczyk,Małgorzata Robak,Waldemar Rymowicz,Zbigniew Lazar
出处
期刊:Sustainability
[MDPI AG]
日期:2020-09-08
卷期号:12 (18): 7364-7364
被引量:7
摘要
Yarrowia lipolytica is a non-conventional yeast producing valuable compounds, such as citric acids, from renewable raw materials. This study investigated the impact of citrate synthase overexpression on the biosynthesis of citric and isocitric acid in Y. lipolytica. Two transformants of Y. lipolytica A101.1.31 strain (efficient citric acid producer), overexpressing CIT1 or CIT2 gene (encoding proteins with citrate synthase activity), were constructed. The results revealed that overexpression of either of these genes enhances citrate synthase activity. Additionally, the cit1 knockout strain was unable to use propionate as the sole carbon source, which proves that CIT1 gene encodes a dual activity protein–citrate and 2-methylcitrate synthase. In the overexpressing mutants, a significant increase in isocitric acid biosynthesis was observed. Both CIT1 and CIT2 overexpressing strains produced citric and isocitric acid from vegetable oil in a ratio close to 1 (CA/ICA ratio for wild-type strain was 4.12).
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