Non-template synthesis of porous carbon nanospheres coated with a DNA-cross-linked hydrogel for the simultaneous imaging of dual biomarkers in living cells

We designed a dual-color fluorescent nanoprobe for simultaneous detection and imaging of adenosine triphosphate and glutathione in living cells based on porous carbon nanospheres and a DNA hybrid hydrogel. Due to the non-template synthesis process and good biocompatibility, the nanohydrogel displayed a short preparation time and anti-nonspecific adsorption.