Study of cryptotanshinone on improving hyperandrogenism of polycystic ovary syndrome via down-regulating the expression of gene CYP17 and AR

Objective: Through studying the effect of cryptotanshinone on hyperandrogenism of polycystic ovary syndrome(PCOS) and possible mechanisms to provide scientific basis for its clinical application. Methods: ①Study on the ef ect of cryptotanshinone on reproductive endocrine function in hyperandrogenism model rats in vivo; ②Study on the inl uence of cryptotanshinone on androgen secretion in hyperandrogenism model cell strain in vitro; ③Study on the possible mechanisms of cryptotanshinone on hyperandrogenism of PCOS by detecting the expression of relvant proteins and the coded genes with immunohistochemistry(IHC) and real-time PCR. Results: After cryptotanshinone intervention, the estrous cyclicity, body weight, ovarian coefficient and ovarian morphology of PCOS model rats had been improved and the serum levels of testosterone(T), androstenedione(A2), luteinizing hormone(LH), sexual binding globulin(SHBG) had been reversed; The secretion of T of PCOS model cell had been suppressed. The possible regulatory mechanisms of cryptotanshinone on improving hyperandrogenism of PCOS might be related with down-regulating the expression of gene CYP17 and AR. Conclusion: Cryptotanshinone may have potential therapeutic ef ect on hyperandrogenism of PCOS patients and deserves further investigation.