This paper studied the influence of density of hydrochloric acid(A),dosage of hydrochloric acid(B),extraction temperature(C) and extraction time(D) on the dissolution rate of calcium from eel bone.Based on this experiment,the result of orthogonal experiment showed the optimal combination is A2B3C2D3,viz.the density of hydrochloric acid was 3 mol/L,the dosage of hydrochloric acid was 4 mL,the extraction temperature was 50 ℃,the extraction time was 150 minutes,the dissolution rate of calcium from eel bone is 81.31 %.The primary and secondary factors affect the dissolution rate of calcium in the order: the density of hydrochloric acid the dosage of hydrochloric acid the extraction temperature the extraction time.