Identification and characterization of cow's milk proteins from the rat intestinal lymph using a proteomic strategy

PROTEOMICSVolume 13, Issue 17 p. 2649-2656 Research Article Identification and characterization of cow's milk proteins from the rat intestinal lymph using a proteomic strategy Xundou Li, Xundou Li National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorLilong Wei, Lilong Wei National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorLulu Jia, Lulu Jia National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorMenglin Li, Menglin Li National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorLisi Zhu, Lisi Zhu National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorLiu Liu, Liu Liu National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorYouhe Gao, Corresponding Author Youhe Gao National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China Correspondence: Professor Youhe Gao, National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing 100005, China E-mail:[email protected] Fax: +86-10-6521-2284Search for more papers by this author Xundou Li, Xundou Li National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorLilong Wei, Lilong Wei National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorLulu Jia, Lulu Jia National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorMenglin Li, Menglin Li National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorLisi Zhu, Lisi Zhu National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorLiu Liu, Liu Liu National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaSearch for more papers by this authorYouhe Gao, Corresponding Author Youhe Gao National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China Correspondence: Professor Youhe Gao, National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing 100005, China E-mail:[email protected] Fax: +86-10-6521-2284Search for more papers by this author First published: 08 July 2013 https://doi.org/10.1002/pmic.201300097Citations: 3 Colour Online: See the article online to view Figs. 1–3 in colour. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Food proteins were considered to be absorbed into the body after being digested to amino acids, dipeptides, and tripeptides. However, there are studies indicating that some proteins can pass through the intestinal epithelium under normal physiological conditions, perhaps not in sufficient quantities to be of nutritional importance, but in quantities that may be antigenically or biologically active. In the present study, rat intestinal lymph samples were collected using a modified lymph fistula rat model in fasting and cow's milk postprandial states. Low molecular weight proteins were enriched by ultrafiltration and differential solubilization, separated by 1D-SDS-PAGE, digested in-gel based on molecular weight, and identified using nano-LC-MS/MS. In the postprandial rat intestinal lymph, nine bovine-specific proteins (false discovery rate ≤1%) were identified in different molecular weight regions. Most proteins identified in lymph were highly abundant proteins in the milk, such as β-lactoglobulin and caseins. Seven of the nine identified bovine-specific proteins are allergens in milk. This strategy can be used to search for proteins that can enter the intestinal lymph and analyze their common features. 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Pharmacol. 2011, 668(Suppl 1), S16– S32. Citing Literature Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description pmic7515-sup-0001-TableS1.xlsx9.7 KB Table 1. Bovine-specific proteins identified in the samples enriched by DS. pmic7515-sup-0002-TableS2a.docx453 KB Table 2A. Bovine-specific proteins identified in the samples enriched by ultrafiltration in rat 1. pmic7515-sup-0003-TableS2b.xlsx12.9 KB Table 2B. Bovine-specific proteins identified in the samples enriched by ultrafiltration in rat 2. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. 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